9T2F
Crystal structure of human PRKCBP1 Zinc finger MYND-type containing 8 with pentaglutamate tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.040, 72.090, 68.670 |
| Unit cell angles | 90.00, 127.02, 90.00 |
Refinement procedure
| Resolution | 54.830 - 1.680 |
| R-factor | 0.2134 |
| Rwork | 0.212 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.900 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.830 | 1.850 |
| High resolution limit [Å] | 1.680 | 1.800 |
| Rmerge | 0.085 | 0.538 |
| Number of reflections | 47262 | 2841 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 25% PEG3350 0.2M sodium chloride 0.1M bis-tris pH 5.5 |
