9T29
Jumonji domain-containing protein 2D with crystallization epitope mutation Q41R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98010 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.167, 73.167, 134.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.320 - 2.013 |
| R-factor | 0.2263 |
| Rwork | 0.225 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.791 | |
| Rmeas | 0.061 | |
| Number of reflections | 27244 | 1986 |
| <I/σ(I)> | 21.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Hampton Index H08 0.1M magnesium formate 15% PEG3350 |
