9T28
WspR response regulator with crystallization epitope mutations G306D:G308D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91739 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.120, 45.400, 85.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.615 - 1.330 |
| Rwork | 0.164 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.801 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.615 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.021 | |
| Rmeas | 0.829 | |
| Number of reflections | 37725 | 1400 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 89.6 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M lithium sulfate 25% PEG3350 0.1M bis-tris pH 6.5 |
