9SZ7
CryoEM structure of MraZ in complex with 4 box promoter from Mycoplasma genitalium
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Energy Filter | TFS Selectris X |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.87 |
| Software | crYOLO,PHENIX,RELION,EMReady,cryoSPARC,SerialEM,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
