9SWY
The structure of AfUAP1 in complex with GlcNAc-1P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.834, 136.865, 143.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.540 - 1.720 |
| R-factor | 0.21112 |
| Rwork | 0.210 |
| R-free | 0.25186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.432 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.540 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 115021 | 4713 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 98.2 | |
| Redundancy | 12.7 | |
| CC(1/2) | 0.999 | 0.314 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M sodium acetate, 25% polyethylene glycol (PEG) 3350 |
