9ST8

Structure of IglFC:IglGN complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97697
Spacegroup name	P 31 2 1
Unit cell lengths	187.220, 187.220, 113.052
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.280 - 3.300
R-factor	0.2168
Rwork	0.215
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.582
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.740	3.460
High resolution limit [Å]	3.300	3.300
Rmerge	0.065	1.251
Rmeas	0.068	1.313
Rpim	0.021	0.398
Total number of observations	357712	49501
Number of reflections	34678	4571
<I/σ(I)>	18.3	1
Completeness [%]	100.0
Redundancy	10.3	10.8
CC(1/2)	0.999	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6 M LiSO4 0.05 M MgSO4 0.05 M HEPES pH7