9SS3

Crystal structure hASF1A 156-cr7

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 41 21 2
Unit cell lengths	52.772, 52.772, 149.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.870 - 1.999
R-factor	0.1921
Rwork	0.190
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.990
Data reduction software	XDS ((VERSION Feb 5, 2021 BUILT=20210205))
Data scaling software	XDS ((VERSION Feb 5, 2021 BUILT=20210205))
Phasing software	MOLREP (11.9.02; 28.02.2022)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.110	2.050
High resolution limit [Å]	1.990	1.990
Rmerge	0.171	2.630
Rmeas	0.175	2.732
Rpim	0.035	0.729
Number of reflections	15054	1073
<I/σ(I)>	14.8	0.9
Completeness [%]	99.9
Redundancy	24.1
CC(1/2)	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Sodium fluoride 20 %(w/v) PEG 3350