9SRK
Structure of collectin-11 (CL-11) carbohydrate-recognition domain in complex with L-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.090, 105.760, 45.320 |
| Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
| Resolution | 45.210 - 1.800 |
| R-factor | 0.1604 |
| Rwork | 0.158 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.954 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.810 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 27248 | 1366 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 94.1 | |
| Redundancy | 1.6 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-acetate pH 8.5 containing 25% PEG High Smear. Crystals were transferred to reservoir solution containing 15% (vol/vol) L-fucose (0.9 M), and incubated for 10 minutes |
