9SRJ

Prenylated FMN oxidative maturase PhdC, PEG-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	I 2 3
Unit cell lengths	121.253, 121.253, 121.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.340 - 1.420
R-factor	0.1819
Rwork	0.181
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.868
Data scaling software	AutoProcess
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	1.440
High resolution limit [Å]	1.420	1.420
Number of reflections	55801	2807
<I/σ(I)>	26.2	0.4
Completeness [%]	100.0	100
Redundancy	37	16.5
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	0.1 M Sodium acetate, pH 4.6, 8 % w/v PEG 4000