9SPU
Echovirus 18 particle missing one pentamer in situ, symmetrized reconstruction (C5)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Phase Plate | OTHER |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.5 |
| Software | cryoSPARC,PHENIX,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
