9SOW
BepA-BamABCDE-OMP-Nb5 complex in open, substrate-engaged beta barrel conformation.
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.55 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.23 |
| Number of Particle | 10108143 |
| Software | SPHIRE,cryoSPARC,PHENIX |
| CTF correction | NONE |
