9SOH

CTLH-CRA domains of the Maea-RanBP10 mutant (Q519G, F543L, Y548F and V556F) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-21
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.9763
Spacegroup name	F 41 3 2
Unit cell lengths	359.825, 359.825, 359.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.850 - 3.545
R-factor	0.2831
Rwork	0.282
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.810
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.084	3.774
High resolution limit [Å]	3.545	3.545
Rmerge	0.282	4.684
Rmeas	0.283	4.698
Rpim	0.023	0.368
Number of reflections	21897	1288
<I/σ(I)>	27.3	2.3
Completeness [%]	95.4	53.3
Redundancy	158.5
CC(1/2)	1.000	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris 8.0, 0.2 M sodium chloride, 20% PEG 6k