9SOC
Murine RanBP10-Twa1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-09 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 124.920, 124.920, 479.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.180 |
| R-factor | 0.2998 |
| Rwork | 0.299 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.307 | 3.788 |
| High resolution limit [Å] | 3.175 | 3.175 |
| Rmerge | 0.367 | 3.891 |
| Rmeas | 0.373 | 3.950 |
| Rpim | 0.069 | 0.670 |
| Number of reflections | 11177 | 559 |
| <I/σ(I)> | 9.3 | 1.3 |
| Completeness [%] | 88.7 | 49.1 |
| Redundancy | 28.1 | 33.8 |
| CC(1/2) | 0.999 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M BisTris 6.0, 0.02 M hexaamminecobalt chloride, 0.18 magnesium chloride, 9% PEG 3350 |
