9SNV

CTLH-CRA domains of the RanBP9-muskelin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-20
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.9763
Spacegroup name	P 61 2 2
Unit cell lengths	84.086, 84.086, 228.282
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.919 - 1.965
R-factor	0.2294
Rwork	0.228
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.548
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.919	2.150
High resolution limit [Å]	1.965	1.965
Rmerge	0.188	6.557
Rmeas	0.191	6.645
Rpim	0.030	1.074
Number of reflections	24304	1215
<I/σ(I)>	16.9	0.8
Completeness [%]	94.9
Redundancy	39
CC(1/2)	0.999	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris 8.5, 0.01 M nickel chloride, 30% PEG 400