9SNO

VHL/Elongin B/Elongin C complex with compound 59

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9121
Spacegroup name	P 41 2 2
Unit cell lengths	94.433, 94.433, 366.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.220 - 2.800
R-factor	0.2583
Rwork	0.256
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.611
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.220	2.910
High resolution limit [Å]	2.800	2.800
Rmerge	0.129	0.687
Rmeas	0.140	0.741
Rpim	0.054	0.276
Number of reflections	42234	4334
<I/σ(I)>	14.5	3.8
Completeness [%]	100.0	100
Redundancy	12.9
CC(1/2)	0.998	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	100 mM Sodium Citrate pH 6.0, 200 mM Magnesium Acetate, 50 mM DTT, 14% PEG 8000