9SNH

CTLH-CRA domain of murine Maea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	62.075, 76.181, 108.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.590 - 1.430
R-factor	0.2003
Rwork	0.199
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.816
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.122	1.583
High resolution limit [Å]	1.430	1.431
Rmerge	0.090	2.855
Rmeas	0.093	2.974
Rpim	0.026	0.822
Number of reflections	67576	3379
<I/σ(I)>	12.9	1
Completeness [%]	94.1
Redundancy	13.3
CC(1/2)	0.998	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M sodium acetate pH 4.6, 0.2 M sodium acetate, 23% PEG 4k