9SNG

CTLH-CRA domain of murine RanBP10 mutant Q519G, F543L, Y548F and V556F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-20
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.9763
Spacegroup name	P 2 21 21
Unit cell lengths	52.079, 68.629, 76.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.040 - 1.740
R-factor	0.212
Rwork	0.210
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.725
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.042	1.870
High resolution limit [Å]	1.737	1.737
Rmerge	0.153	1.979
Rmeas	0.159	2.054
Rpim	0.043	0.545
Number of reflections	23475	1201
<I/σ(I)>	13.1	1.5
Completeness [%]	91.7	50.7
Redundancy	13.4
CC(1/2)	0.999	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M BisTris 6.5, 30% PEG 400