9SNF
CTLH-CRA domain of murine RanBP10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 55.331, 75.500, 76.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.530 - 2.070 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.369 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 2.294 |
| High resolution limit [Å] | 2.069 | 2.069 |
| Rmerge | 0.166 | 1.797 |
| Rmeas | 0.174 | 1.882 |
| Rpim | 0.051 | 0.552 |
| Number of reflections | 14606 | 859 |
| <I/σ(I)> | 11.5 | 1.7 |
| Completeness [%] | 91.3 | 57.4 |
| Redundancy | 11.3 | 11.1 |
| CC(1/2) | 0.995 | 0.602 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.1 M tris pH 7.8, 15% PEG 3350 |
