9SM1

FPP-Methyltransferase from Pseudomonas chlororaphis in complex with SAH and FPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	261.430, 100.040, 89.540
Unit cell angles	90.00, 98.03, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.18595
Rwork	0.184
R-free	0.21739
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.225
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.089	0.738
Number of reflections	111920	13727
<I/σ(I)>	11.1	2.7
Completeness [%]	96.5	95.3
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	1.4 M Na/K-Phosphate