9SM0
FPP-Methyltransferase from Burkholderia territorii in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0597 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 108.630, 108.630, 73.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.14651 |
| Rwork | 0.145 |
| R-free | 0.16830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.391 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.066 | 0.781 |
| Number of reflections | 85663 | 15903 |
| <I/σ(I)> | 13.6 | 2.3 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 25% PEG 3350, 0.2 M MgCl2 |
