9SLZ

Structure of Thermus thermophilus lysyl-tRNA synthetase complexed with wild-type E.coli tRNALys(mnm5s2UUU) and sulphamoyl-analogue of lysyl-adenylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-03-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.885
Spacegroup name	P 31 2 1
Unit cell lengths	147.300, 147.300, 127.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.929 - 3.800
Rwork	0.212
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.745
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.900
High resolution limit [Å]	3.800	3.800
Rmerge	0.104	0.366
Number of reflections	15932	1094
<I/σ(I)>	6.02
Completeness [%]	99.1
Redundancy	3.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	24-26% saturated ammonium sulphate. 50 mM Tris-maleate (pH 7.6), 10 mM MgCI2, 1 mM NaN3, 4 mg/ml LysRSTT, 2.5 mg/ml wt Ecoli tRNALys and 325 microM LysAMS. Cryoprotectant 30% glycerol.