9SLQ
Trypanosome brucei enolase in complex with a camelid single-domain antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.640, 79.020, 161.330 |
| Unit cell angles | 90.00, 106.68, 90.00 |
Refinement procedure
| Resolution | 47.090 - 2.330 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.090 | 2.350 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmeas | 0.157 | |
| Number of reflections | 101302 | 16191 |
| <I/σ(I)> | 6.42 | 0.69 |
| Completeness [%] | 98.1 | 97.8 |
| Redundancy | 3.56 | 3.63 |
| CC(1/2) | 0.993 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 4000, 10% 2-propanol, 100 mM tri-sodium citrate pH 5.6 |
