9SLB
Zuzalysin active bi-pentamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-10 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 129.800, 129.400, 188.500 |
| Unit cell angles | 98.20, 104.70, 115.10 |
Refinement procedure
| Resolution | 84.160 - 3.000 |
| R-factor | 0.2567 |
| Rwork | 0.257 |
| R-free | 0.29630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.527 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0rc1_5617) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.160 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.172 | |
| Rmeas | 0.206 | |
| Number of reflections | 200908 | 998 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium dihydrogen phosphate 1.2M, Di-potasium hydrogen phosphate 0.8M, Lithium sulfate anhydre 0.2M, CAPS 0.1M pH 10.5 |
