9SJT
Cryo-EM structure of Human Apoferritin at pH 5
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.96 |
| Number of Particle | 521922 |
| Software | Topaz,PHENIX,RELION,cryoSPARC,EPU,Gctf,UCSF ChimeraX,Coot |
| Symmetry | O |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
