9SJP
Single-chain chimeric protein mimicking the interaction between HR1 and HR2 in HIV gp41 bound to ANS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.500, 53.100, 58.000 |
| Unit cell angles | 70.02, 69.93, 71.65 |
Refinement procedure
| Resolution | 19.470 - 1.150 |
| R-factor | 0.1508 |
| Rwork | 0.150 |
| R-free | 0.17270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.266 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.470 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.031 | 0.520 |
| Rmeas | 0.043 | 0.733 |
| Rpim | 0.031 | 0.518 |
| Number of reflections | 154137 | 6335 |
| <I/σ(I)> | 12.5 | 1.7 |
| Completeness [%] | 88.9 | 73.6 |
| Redundancy | 1.8 | 1.6 |
| CC(1/2) | 0.999 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M ammonium sulfate, 30% w/v PEG 8000, 0.1 M MES |
