9SJG

Crystal structure of apo GHdex dextranase (BT3087), wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.89842
Spacegroup name	P 41 21 2
Unit cell lengths	93.698, 93.698, 331.612
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.133 - 1.800
Rwork	0.198
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.815
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.270	55.270	1.830
High resolution limit [Å]	1.800	9.860	1.800
Rmerge	0.161	0.035	2.239
Rmeas	0.167	0.036	2.323
Rpim	0.046	0.009	0.618
Number of reflections	137723	1035	6704
<I/σ(I)>	13.4	41.4	1.6
Completeness [%]	100.0	99.5	100
Redundancy	25.1	23.1	27.3
CC(1/2)	0.999	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MES 0.8-3.2M ammonium sulphate