9SID

Crystal structure of human Signal Regulatory Protein 2 (SIRP) alpha V2 - Q52F mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980103289881
Spacegroup name	P 21 21 21
Unit cell lengths	57.535, 58.049, 66.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.800 - 1.690
R-factor	0.2409
Rwork	0.239
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.961
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.690	1.690
Number of reflections	25431	4008
<I/σ(I)>	9.95	0.89
Completeness [%]	99.8	98.9
Redundancy	12.3	9.39
CC(1/2)	0.997	0.285

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.01M Zinc sulfate heptahydrate 0.1M MES monohydrate pH 6.5 25% v/v PEG monomethyl ether 550