9SIC

Crystal structure of human Signal Regulatory Protein 2 (SIRP) alpha V2 - Q52A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565037251
Spacegroup name	P 41 21 2
Unit cell lengths	57.287, 57.287, 200.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.510 - 1.500
R-factor	0.18089
Rwork	0.179
R-free	0.21625
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.325
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	54492	8625
<I/σ(I)>	32.64
Completeness [%]	100.0
Redundancy	27.7
CC(1/2)	0.999	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.01M Zinc sulfate heptahydrate 0.1M MES monohydrate pH 6.5 25% v/v PEG monomethyl ether 550