9SIA
Crystal structure of human Signal Regulatory Protein 2 (SIRP) alpha V2 in complex with L-Tryptophane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.355, 57.355, 198.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.320 - 1.830 |
| R-factor | 0.2189 |
| Rwork | 0.217 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.320 | 1.890 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 29981 | 2905 |
| <I/σ(I)> | 14 | 1.8 |
| Completeness [%] | 99.0 | |
| Redundancy | 7.3 | |
| CC(1/2) | 0.998 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.01M Zinc sulfate heptahydrate 0.1M MES monohydrate pH 6.5 25% v/v PEG monomethyl ether 550 |
