9SI7

Crystal structure of TBC domain of human TBC1D17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 32
Unit cell lengths	97.977, 97.977, 82.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.000 - 3.340
R-factor	0.22392
Rwork	0.215
R-free	0.28677
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	3.630
High resolution limit [Å]	3.340	3.340
Number of reflections	8626	432
<I/σ(I)>	13.54
Completeness [%]	92.5
Redundancy	10.7
CC(1/2)	0.999	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG Smear Broad, 150 mM Li2SO4, 0.1 M HEPES pH 7.0