9SI6

Crystal structure of TBC domain of murine TBC1D17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 2
Unit cell lengths	94.241, 160.895, 41.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.660 - 2.200
R-factor	0.183
Rwork	0.179
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.978
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.660	2.330
High resolution limit [Å]	2.200	2.200
Number of reflections	32171	5195
<I/σ(I)>	8.03
Completeness [%]	96.7
Redundancy	3.44
CC(1/2)	0.996	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	30% Precipitant mix (20% PEG 3350, 20% PEG 1000, 20% MPD), 50 mM MgCl2, 50 mM CaCl2, 0.1M Tris pH 8.5