9SH8
Thermus thermophilus asparaginyl-tRNA synthetase with bound sulphamoyl-analogue of asparaginyl-adenylate (AsnAMS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-15 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.890 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 125.183, 125.183, 126.591 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.840 - 2.620 |
| Rwork | 0.209 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.061 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.840 | 2.690 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.052 | 0.257 |
| Number of reflections | 16965 | 1073 |
| <I/σ(I)> | 9.54 | |
| Completeness [%] | 94.5 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | Native crystals grown with protein 5 mg ml- l, 12-15 % PEG 6000, 0.3M KCI at pH 8.4. Soaked for 15 h in mother liquor containing 100 mM AsnAMS. |
