9SG2
Ixekizumab Fab in complex with human IL-17A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 125.730, 125.730, 107.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.720 - 2.920 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.910 | 3.000 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmeas | 0.163 | 2.972 |
| Number of reflections | 19277 | 1400 |
| <I/σ(I)> | 11.99 | 1.02 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.55 | 13.35 |
| CC(1/2) | 0.999 | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M TRIS pH 8.5, 40% MPD |
