9SFM

Crystal structure of Cereblon-DDB1 in complex with SB-405483 and Lenalidomide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	72.160, 129.518, 198.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.400 - 2.390
R-factor	0.197
Rwork	0.195
R-free	0.24410
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.520	2.440
High resolution limit [Å]	2.390	2.390
Rmerge	0.112	1.228
Number of reflections	74800	7356
<I/σ(I)>	16.6	2.1
Completeness [%]	99.9	97.8
Redundancy	13.5	12.8
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% w/v PEG 4000 0.1 M TRIS pH 8.2, 0.2 M