9SDJ

Complex of Ovine Serum Albumin with Hydrolyzed Ampicillin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 32 2 1
Unit cell lengths	121.189, 121.189, 121.965
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.200 - 2.550
R-factor	0.16587
Rwork	0.164
R-free	0.21262
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.325
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.550	2.550
Rmerge	0.081
Number of reflections	32992	4387
<I/σ(I)>	15.01	1.46
Completeness [%]	96.7	80.7
Redundancy	6.33
CC(1/2)	0.999	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	80% Tacsimate pH 7.25 and 2% of PEG 400