9SD5

Crystal structure of the C-terminally truncated human E574Q-PGGHG mutant in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	54.071, 77.076, 79.888
Unit cell angles	90.00, 98.55, 90.00

Refinement procedure

Resolution	79.000 - 1.363
Rwork	0.136
R-free	0.15730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.021
Data scaling software	Aimless (0.7.13)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.000	1.390
High resolution limit [Å]	1.360	1.360
Rpim	0.027	0.296
Number of reflections	137926	6833
<I/σ(I)>	20.2	3.4
Completeness [%]	99.9	99.9
Redundancy	6.6	5.6
CC(1/2)	0.999	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.7	289	Ratio: 1:1 Co-crystallized with 10mM KJB Conditions: 20% PEG 3350, 0.1 M Tris pH 8.7, 0.2 M NaCl.