9SCE
Structure of S. pombe PNUTS (565 - 644) bound to Swd2.2 - crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-26 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.770, 93.296, 81.871 |
| Unit cell angles | 90.00, 90.89, 90.00 |
Refinement procedure
| Resolution | 50.920 - 1.610 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.739 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.861 | 1.740 |
| High resolution limit [Å] | 1.605 | 1.605 |
| Rmerge | 0.132 | 0.952 |
| Rmeas | 0.143 | 1.087 |
| Rpim | 0.054 | 0.515 |
| Number of reflections | 92275 | 4614 |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 94.7 | 59.7 |
| Redundancy | 6.8 | 4.2 |
| CC(1/2) | 0.998 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 10% w/v PEG 8000, 20% v/v ethylene glycol 0.03 M of each ethylene glycol 0.1M MES/imidazole pH 6.5 |
