9SAI

Ternary PROTAC-mediated complex consisting of Cereblon, DDB1 and BRD4-BD1, non-covalently linked by JQ1-AcN

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Energy Filter	TFS Selectris X
Detector	TFS FALCON 4i (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.66
Software	cryoSPARC,PHENIX,EPU,UCSF ChimeraX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION