9SA2

The RING domain of IDOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9405
Spacegroup name	P 1 21 1
Unit cell lengths	44.190, 47.610, 70.540
Unit cell angles	90.00, 90.86, 90.00

Refinement procedure

Resolution	70.532 - 1.100
Rwork	0.142
R-free	0.17410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.414
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.540	1.120
High resolution limit [Å]	1.100	1.100
Rmerge	0.492
Rmeas	0.503	12.512
Rpim	0.101	3.316
Number of reflections	118793	5872
<I/σ(I)>	8.9	0.7
Completeness [%]	100.0	100
Redundancy	49.4	27.5
CC(1/2)	0.998	0.314

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	200 mM Sodium acetate, 100 mM Tris, 30% w/v PEG 4000
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	200 mM Sodium acetate, 100 mM Tris, 30% w/v PEG 4000