9S9G
S. islandicus CdvA filament (X-ray)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.032, 102.716, 328.554 |
| Unit cell angles | 90.00, 92.35, 90.00 |
Refinement procedure
| Resolution | 52.620 - 2.850 |
| R-factor | 0.2299 |
| Rwork | 0.226 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.432 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 325.960 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 1.666 | |
| Rmeas | 1.711 | 16.086 |
| Rpim | 0.358 | 3.780 |
| Total number of observations | 4796820 | |
| Number of reflections | 183198 | 25112 |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 98.5 | |
| Redundancy | 26.2 | 19.1 |
| CC(1/2) | 0.672 | 0.137 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.282 M KH2PO4, 0.094 M Tris/acetate |
