9S9C

Crystal structure of the apo BRL2 ectodomain from Arabidopsis thaliana.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.999879
Spacegroup name	P 41 21 2
Unit cell lengths	118.613, 118.613, 155.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.530 - 2.590
R-factor	0.2687
Rwork	0.267
R-free	0.29190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.530	2.760
High resolution limit [Å]	2.590	2.590
Rmeas	0.110	2.373
Number of reflections	34843	5378
<I/σ(I)>	20.3	1.53
Completeness [%]	99.5	97.4
Redundancy	25.2	21.3
CC(1/2)	1.000	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	23 % PEG 3,350, 0.2 M MgCl2 6H2O, 0.1 M Tris pH 9.0, 20% glycerol