9S93
Strucutre of human transthyretin mutant I68L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 VENTURE |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-31 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 41.690, 84.740, 63.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.290 - 1.940 |
| R-factor | 0.25819 |
| Rwork | 0.258 |
| R-free | 0.27061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.290 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.110 | 0.470 |
| Number of reflections | 17062 | 2628 |
| <I/σ(I)> | 7.7 | 2.1 |
| Completeness [%] | 98.7 | 95.5 |
| Redundancy | 3.9 | |
| CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 0.4 M CaCl2, 34% PEG400 |
