9S8V
Crystal structure of the BRI1 ectodomain from Arabidopsis thaliana in complex with 24-epibrassinolide.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.454, 66.605, 119.754 |
| Unit cell angles | 90.00, 121.43, 90.00 |
Refinement procedure
| Resolution | 47.900 - 2.400 |
| R-factor | 0.2494 |
| Rwork | 0.248 |
| R-free | 0.27300 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.069 | 2.488 |
| Number of reflections | 312324 | 7056 |
| <I/σ(I)> | 13.7 | 1 |
| Completeness [%] | 98.5 | 96.4 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 1.000 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 23% PEG 3350, 0.2 M Li2SO4, 0.1 M citric acid pH 4.0, 25% ethylene glycol |
