9S87
Crystal structure of the BRI1 ectodomain from Arabidopsis thaliana in complex with castasterone.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-28 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.404, 66.897, 120.434 |
| Unit cell angles | 90.00, 122.46, 90.00 |
Refinement procedure
| Resolution | 47.870 - 2.140 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.24310 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.679 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmeas | 0.077 | 2.270 |
| Number of reflections | 65117 | 10343 |
| <I/σ(I)> | 11.7 | 1 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 7 | 7 |
| CC(1/2) | 1.000 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 24% PEG 3350, 0.2M Li2SO4, 0.1M citric acid pH 4.0, 25% ethylene glycol |
