9S81
Crystal structure of the LEDGF PWWP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.873128 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.284, 36.770, 76.667 |
| Unit cell angles | 90.00, 114.10, 90.00 |
Refinement procedure
| Resolution | 69.980 - 1.630 |
| R-factor | 0.22902 |
| Rwork | 0.227 |
| R-free | 0.26951 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.659 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.980 | 1.710 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.072 | 3.330 |
| Rmeas | 0.079 | 3.590 |
| Rpim | 0.030 | 1.332 |
| Total number of observations | 266164 | 39296 |
| Number of reflections | 38383 | 5523 |
| <I/σ(I)> | 8.3 | 0.6 |
| Completeness [%] | 99.5 | |
| Redundancy | 6.9 | 7.1 |
| CC(1/2) | 0.999 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 400 0.03 M CAPS pH 10.5 |
