9S73
Complex of Equine Serum Albumin with Ampicillin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 61 |
| Unit cell lengths | 94.960, 94.960, 143.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.530 - 2.500 |
| R-factor | 0.20362 |
| Rwork | 0.201 |
| R-free | 0.26348 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 25271 | 2778 |
| <I/σ(I)> | 18.88 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.76 | 5.8 |
| CC(1/2) | 0.999 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 2.0 M ammonium sulfate, 0.1 M acetate buffer pH 5.0 |
