Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.721, 93.721, 141.217 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.860 - 2.030 |
| R-factor | 0.16191 |
| Rwork | 0.161 |
| R-free | 0.20636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.937 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.860 | 2.130 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 45127 | 6033 |
| <I/σ(I)> | 21.83 | 2.12 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.57 | |
| CC(1/2) | 0.999 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 2.0 M ammonium sulfate, 0.1 M acetate buffer at pH 5.0 and then crystal was cryoprotected by 85% Tacsimate at pH 6.0 |
