9S3L
ClxA from Clostridium cavendishii (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2023-04-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.73380 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.940, 79.970, 81.770 |
| Unit cell angles | 90.26, 95.58, 103.50 |
Refinement procedure
| Resolution | 40.710 - 2.570 |
| R-factor | 0.26687 |
| Rwork | 0.265 |
| R-free | 0.30094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 2.640 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.290 | 1.180 |
| Rpim | 0.170 | 0.760 |
| Number of reflections | 55692 | 4566 |
| <I/σ(I)> | 2.8 | 0.4 |
| Completeness [%] | 99.2 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.960 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M MgCl2; 0.1 M Tris-HCl; 25% (w/v) PEG 3350 |
