9S28
MVV STC intasome in complex with LEDGF
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | TFS Selectris |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.8 |
| Number of Particle | 60999 |
| Software | Topaz,RELION,cryoSPARC,UCSF Chimera,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
