9S1A
WRN helicase in the absence of nucleotide or DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.760, 80.240, 83.090 |
| Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
| Resolution | 57.720 - 1.956 |
| R-factor | 0.2088 |
| Rwork | 0.207 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.719 | 2.005 |
| High resolution limit [Å] | 1.956 | 1.956 |
| Rmerge | 0.075 | 0.030 |
| Rmeas | 0.081 | 1.134 |
| Rpim | 0.031 | 0.633 |
| Number of reflections | 60721 | 3036 |
| <I/σ(I)> | 15.94 | 1.8 |
| Completeness [%] | 90.3 | 70.4 |
| Redundancy | 6.69 | 6.1 |
| CC(1/2) | 0.999 | 0.423 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 100mM BIS-TRIS pH 5.8, 25% w/v PEG3350 and 200mM LiSO4. |
